新西兰环球理工学院论文代写: 膜片钳系统

新西兰环球理工学院论文代写: 膜片钳系统

膜片钳系统用于测量细胞膜中离子通道的电流,这是发现药物的重要细胞。然而,用玻璃微电极片蛤的常规系统被称为是繁琐以及低水平的吞吐量(Rasooly,2009c)。为了克服这些缺点,人们提出了基于芯片的膜片钳技术,用于研究活细胞中离子通道。这种特殊的系统可以用作在高吞吐量标准系统中进行膜片钳的横向方法。此外,该系统可以被轻松地集成,被称为自然的强大的微流体器件的方法(Rasooly,2009)。这些可以帮助管理时空对药物相关的不同剂量。混合动力系统是由一块硅芯片组成,它集成了12个玻璃毛细管,用于固定和修补电池,以及与具有一定数量孔的芯片粘合的聚二甲基硅氧烷层。该系统有助于提供压力两种不同来源以及一系列八行动控制实验室-操作-芯片系统(rasooly,2009b)。压力的来源之一是在芯片上的弹性阀的操作上设置10 psi的最大水平为20 psi,而另一个已知的设置在5 psi以下以驱动液体的流动。
在一些商用系统,液滴微流控芯片已经演变为在一个强大的生化操作系统。这是由于这样的事实,有需要的只有特定的卷皮-纳升级样品。在这些特殊的系统,插头或液滴可以产生他们将样品溶液在水溶性载体如气体或疏水性化合物中(Rasooly,2009)。然而,的单分散性,频率的精确控制,形状和液滴大小仍有助吸引相关的重大价值的重视和高可靠的吞吐量分析相关。

新西兰环球理工学院论文代写: 膜片钳系统

Patch- clamp systems are used for the measurement of currents across the channels of ion in the membranes of cells are important cells in the discovery of drug. However, the conventional systems of patch clams using the micropipettes of glass are known to be cumbersome as well as low level of throughput (Rasooly, 2009c). For overcoming these disadvantages, there has been presentation of chip based devices of patch clamp that had been designed for studying the channels of ion in lively cells. This particular system can be used as a lateral approach of patch clamping within a standard system of high- throughput. In addition to this, this system helps the method for being integrated easily in the microfluidic devices that are known to be robust in nature (Rasooly, 2009a). These can help in administering the different doses of spatiotemporal related to a drug. The hybrid system is known to consist of a chip of silicon that integrates 12 capillaries of glass for the immobilizing and patching the cells, as well as the layer of polydimethylsiloxane bonded with the chip having defined number of holes. This system helps in providing two different sources of pressure along with an array of eight lines of pneumatic control for the operation of lab- on- a- chip system (Rasooly, 2009b). One of the sources of pressure is set more the 10 psi with the maximum level of 20 psi for the operation of elastomeric valves on chip, while the other is known to be set below 5 psi for driving the flow of liquid.
Amongst a number of commercially available systems, the droplet microfluidics has been evolved as a powerful system in the biochemical operations. This is due to the fact that there is a need of only certain volumes from sample of pico- to nanolitre. In these particular systems, plugs or droplets can be generated as they immerse the aqueous samples within the medium of water immiscible carrier like gas or hydrophobic compound (Rasooly, 2009a). However, accurate control of the monodispersity, frequency, shape and size related to the droplets still help in attracting a significant value of attention related to high and reliable throughput assays.