CD24 is a small cell surface protein molecule that has binded to the glycosylphosphatidylinositol in many kinds of cancer . It is has been found that they function as a signal transducer. It is otherwise known as the cluster of differencation 24 or heat stable antigen. CD24 (HSA)  is actually a protein that is encoded by the CD24 gene. This is found on the chromosome 6. Heavily glycosylated cell surface protein CD24 is important in the study of breast cancer. CD24+ cancer cells tend to spread more easily in bloodstream and seed new colonies in secondary organs [9, 10]. CD24 glycoprotein is found in the lymphocytes and some kinds of neuroblasts. The protein encoded is found to be adjoined via glycosyl phosphatidylinositol (GPI) to the cell surface. It has been found that the CD24 is often associated with the prognosis of various cancers. The exact heuristics and the mechanism adopted by the CD24 are not clear. CD24 expression was analyzed in 747 breast cancer tissues. It was found that the CD24 was used in the DNA methylation and histone modification status in the promoter region of CD24. It was found that the patients who had higher levels of CD24 had shorter survival rates that with patients who had low CD24 . It was also found from analysis that the gene expression of CD24 was involved in histone acetylation. This is an independent processing the DNA methylation . This points to the mechanism of action of the epigenetic regulation of CD24. The result indicated that over gene expression of the CD24 gene causes lower survival rates .
Thus analysis of CD24 can be a potentially strong therapeutic target for the specific subtypes of breast cancer. CD24 is thus found to be more adept in diagnosing carcinoma owing to their enhanced functionality. It has been found that the glycosylation has diversity in the proteome. Every aspect of the glycosylation process can be changed or modified . This includes the glycoside bond, glycerin composition, glycerin structure and also in the oligosachharide layer. From this analysis, it is found that glycosylation plays an important biochemical process and is involved in a number of biochemical pathways in the body. E-selectin is found to play an important role in this process. The reason for exploring the function of glycosylation and the CD24, CD44 gene expression is to understand the role of the glycosylation for the metastasis. Empirical research has found that glycosylation plays an important role in the metastasis function. Details are discussed in detail in the following.